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A simple and cost‐effective HPLC‐UV method for the detection of levetiracetam in plasma/serum of patients with epilepsy
Author(s) -
Engelbrecht Lynette,
Grobler C. J.,
Rheeders Malie
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3969
Subject(s) - chemistry , chromatography , levetiracetam , high performance liquid chromatography , calibration curve , therapeutic drug monitoring , analyte , correlation coefficient , potassium , analytical chemistry (journal) , detection limit , pharmacokinetics , epilepsy , pharmacology , neuroscience , biology , medicine , statistics , mathematics , organic chemistry
A simple, fast and cost‐effective method was developed and validated for the determination of levetiracetam (LEV) in plasma/serum of patients using high performance liquid chromatography (HPLC) with ultraviolet detection. The stability of LEV plasma/serum samples over time and in different blood collection tubes was evaluated. Serum/plasma samples were deproteinized by methanol spiked with the internal standard, gabapentin. HPLC was carried out on a Venusil XBP C 18 , 250 × 4.6 mm, 5 μm column, at a flow rate of 1.0 mL/min and with mobile phase consisting of 50 m m potassium dihydrogen phosphate–acetonitrile at a pH of 5.5. The UV detector was set at 205 nm and 10 μL was injected. Total runtime was 15 min. Calibration curves were linear (correlation coefficient = 0.999) over a concentration range of 1–60 μg/mL. Relative standard deviation values for both the inter‐day and intra‐day precision and accuracy were <5% for the concentration range. The influence of different collection tubes and the effect of time on the stability of LEV was investigated. These factors may cause inaccuracies owing to drug–protein binding and interference in the matrix. This method is simple, fast, cost‐effective, reliable and accurate with minimal sample preparation for daily routine use in therapeutic drug monitoring.