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Sensitive quantification of coixol, a potent insulin secretagogue, in Scoparia dulcis extract using high‐performance liquid chromatography combined with tandem mass spectrometry and UV detection
Author(s) -
Ali Arslan,
Haq Faraz Ul,
Arfeen Qamar,
Sharma Khaga Raj,
Adhikari Achyut,
Musharraf Syed Ghulam
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3964
Subject(s) - chromatography , chemistry , detection limit , scoparia , tandem mass spectrometry , calibration curve , mass spectrometry , high performance liquid chromatography , liquid chromatography–mass spectrometry , quantitative analysis (chemistry) , zoology , biology
Diabetes is a major global health problem which requires new studies for its prevention and control. Scoparia dulcis , a herbal product, is widely used for treatment of diabetes. Recent studies demonstrate coixol as a potent and nontoxic insulin secretagog from S. dulcis . This study focuses on developing two quantitative methods of coixol in S. dulcis methanol‐based extracts. Quantification of coixol was performed using high‐performance liquid chromatography–tandem mass spectrometry (method 1) and high‐performance liquid chromatography–ultraviolet detection (method 2) with limits of detection of 0.26 and 11.6 pg/μL, respectively, and limits of quantification of 0.78 and 35.5 pg/μL, respectively. S. dulcis is rich in coixol content with values of 255.5 ± 2.1 mg/kg (method 1) and 220.4 ± 2.9 mg/kg (method 2). Excellent linearity with determination coefficients >0.999 was achieved for calibration curves from 10 to 7500 ng/mL (method 1) and from 175 to 7500 ng/mL (method 2). Good accuracy (bias < −8.6%) and precision (RSD < 8.5%) were obtained for both methods. Thus, they can be employed to analyze coixol in plant extracts and herbal formulations.