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Long‐chain unsaturated fatty acids as possible important metabolites for primary angle‐closure glaucoma based on targeted metabolomic analysis
Author(s) -
Rong Shengzhong,
Li Yang,
Guan Yue,
Zhu Lili,
Zhou Qiang,
Gao Mucong,
Pan Hongzhi,
Zou Lina,
Chang Dong
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3963
Subject(s) - chemistry , metabolomics , arachidonic acid , glaucoma , intraocular pressure , linoleic acid , palmitoleic acid , medicine , fatty acid , endocrinology , ophthalmology , chromatography , biochemistry , enzyme
Primary angle‐closure glaucoma (PACG) is a severe chronic neurodegenerative disease in Asia. Identification of important metabolites associated with PACG is crucial for early intervention and advancing knowledge of the disease mechanism. We applied gas chromatography–mass spectrometry (GC‐MS) for targeted metabolomic analysis on serum samples from 38 newly diagnosed PACG patients and 48 controls. Palmitoleic acid (PA), linoleic acid (LA), γ ‐linolenic acid (GLA) and arachidonic acid (ARA) were identified as important metabolites associated with PACG. PA and GLA were significantly elevated ( p < 0.05), while LA and ARA showed a significantly decreased trend ( p < 0.05) in PACG group compared with the control group. Also, significant negative correlations were observed between LA and ARA levels and intraocular pressure of the left eye ( r s = −0.750, p < 0.001; r s = −0.729, p < 0.001) and the right eye ( r s = −0.786, p < 0.001; r s = −0.764, p < 0.001). Serum GLA level was positively correlated with intraocular pressure of the left eye ( r s = 0.233, p = 0.031) and the right eye ( r s = 0.226, p = 0.036). Our findings revealed a significant difference of the serum free fatty acid metabolic profiles between PACG patients and control subjects. Furthermore, PA, LA, ARA and GLA appear to have clinical applications for the screening of PACG.