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Simultaneous quantification of antofloxacin and its major metabolite in human urine by HPLC–MS/MS, and its application to a pharmacokinetic study
Author(s) -
Ma Yunsu,
Wang Qianru,
Zhang Hongwen,
Xie Lijun,
Chen Juan,
Huang Mao,
Liu Yun,
Wang Yuanyuan,
Wang Libin,
Sun Luning,
Ou Ning
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3962
Subject(s) - chemistry , chromatography , metabolite , urine , pharmacokinetics , high performance liquid chromatography , pharmacology , biochemistry , medicine
This study presents a high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method for the simultaneous determination of antofloxacinin and its main metabolite – N ‐demethylated metabolite ( N‐ DM) – in human urine. Ornidazole was used as the internal standard. This was a clinical urine recovery study, in which 10 healthy Chinese volunteers were intravenously administered a single 200 mg dose of antofloxacin hydrochloride. Compounds were extracted by albumen precipitation, after which samples were isocratically eluted using a Poroshell 120 SB‐C 18 column, and were analysed using HPLC–MS/MS under electronic spray ionization positive ion mode. The method was successfully applied in a urine pharmacokinetic study of antofloxacinin, with a detection range of 0.02/0.01 to 200/100 μg/mL (for antofioxacin/ N‐ DM).The average percentages of antofioxacin/ N‐ DM measured in urinary excretion frp, 10 volunteers were 54.9 ± 5.7/8.2 ± 2.5% in 120 h duration.