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Elucidation of stress‐induced degradation products of mangiferin: Method development and validation
Author(s) -
Khurana Rajneet Kaur,
Kaur Satvinder,
Kaur Jasleen,
Singh Bhupinder
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3935
Subject(s) - mangiferin , chemistry , acetic acid , degradation (telecommunications) , chromatography , acetonitrile , organic chemistry , telecommunications , computer science
Abstract The degradation behavior of mangiferin, under various ICH Q1A(R2) recommended stress conditions, was studied using an isocratic elution with mobile phase (pH 2.4), composed of acetonitrile and 1% orthophosphoric acid (12:88 v/v) at a flow rate of 1.0 mL/min, with λ max 262 nm. It was suitably adapted for LC–MS studies by replacing with 1% acetic acid (ACN–1% acetic acid; 18:82) and the pH was adjusted to 3.0. Extensive degradation was found to occur during alkaline medium stress studies at 2.31 min of retention time at λ max of 235 nm. The mass spectrum of mangiferin, 3 h after treatment with 0.1 M NaOH, clearly shows the rupture of the tricyclic ring, indicating that a fragment at m / z − 269 was formed. Furthermore, the results were supported by nuclear magnetic resonance as well. However, no degradation was observed in other stress conditions.