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Quantification of furosemide in camel plasma by high resolution mass spectrometry, application on pharmacokinetics
Author(s) -
Wasfi Ibrahim A.,
Wajid Sayed A.,
Agha B. A.,
Kamel Asmaa M.,
Al Biriki Nasreen A.,
Al Neaimi Khaled M.,
Al Ali Waleed A.
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3902
Subject(s) - chemistry , chromatography , pharmacokinetics , furosemide , mass spectrometry , calibration curve , resolution (logic) , extraction (chemistry) , detection limit , pharmacology , medicine , organic chemistry , artificial intelligence , computer science
We developed and validated a high‐resolution liquid chromatography mass spectrometry method for the quantification of furosemide in camel plasma which was used for a pharmacokinetic study in camels. Plasma samples were extracted by supported liquid extraction and furosemide and internal standard (furosemide‐D5) were separated on a an Agilent Zorbax XDB C 18 column (50 × 2.1 mm i.d., 3.5 μm). Data was acquired in full‐scan mode over a mass range of 200–400 Da in negative electrospray mode at a resolution of 70,000. Linear calibration curves were obtained over the concentration ranges of 1.0–10,000 ng/mL. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolites of furosemide in six camels ( Camelus dromedarus ) and we were able to advice on a withdrawal time of furosemide treatment before racing.