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Application of a solvent‐free solid injection technique coupled with GC–MS for discrimination between the secondary metabolites of wild and cultivated South Korean medicinal foods
Author(s) -
Truong Lieu T. B.,
Abd ElAty A. M.,
Kim Hyun Jin,
Rahman Md. Musfiqur,
Kim SungWoo,
Shin HoChul,
Shim JaeHan
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3896
Subject(s) - furfural , chemistry , phytol , furan , squalene , gas chromatography–mass spectrometry , acetic acid , chromatography , traditional medicine , organic chemistry , biochemistry , mass spectrometry , medicine , catalysis
Solvent‐free solid injection was applied to differentiate between wild and cultivated South Korean medicinal foods, including dureup ( Aralia elata ), deodeok ( Codonopsis lanceolata ) and doraji ( Platycodon grandiflorus ). A number of compounds were identified in wild and cultivated dureup (53 and 46), deodeok (47 and 51) and doraji (43 and 38). Secondary metabolites, including butanal,2‐methyl‐, β ‐caryophyllene, neoclovene, α ‐humulene, γ ‐curcumene, β ‐bisabolene, and phytol, were identified in dureup with significantly ( P < 0.05) different amounts between both types. In deodeok, squalene and other main components such as acetic acid, methyl ester, furan‐methyl‐furfural, 2‐furan‐methanol, and 5‐methyl‐furfural, were statistically different between the two types. Doraji has significantly different compounds such as furfural, 5‐methyl‐furfural, 2‐methoxy‐phenol, 2‐methoxy‐4‐(1‐propenyl)‐phenol, and 1‐(4‐hydroxy‐3‐methoxyphenyl)‐2‐propanone. Although we failed to confirm the key compounds, a new compound, namely desaspidinol, was synthesized for the first time and its retention index determined under the experimental conditions. This solventless, easy technique can be used as a simple way to discriminate between wild and cultivated types of medicinal plants via identification of volatile markers or specific fingerprints.