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Simple extraction method for quantification of phenothiazine residues in pork muscle using liquid chromatography–triple quadrupole tandem mass spectrometry
Author(s) -
Zhang Dan,
Park JinA,
Kim SeongKwan,
Cho SangHyun,
Cho SooMin,
Shim JaeHan,
Kim JinSuk,
Abd ElAty A. M.,
Shin HoChul
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3891
Subject(s) - ammonium formate , chemistry , chromatography , formic acid , mass spectrometry , tandem mass spectrometry , acetonitrile , extraction (chemistry) , detection limit , liquid chromatography–mass spectrometry , triple quadrupole mass spectrometer , formate , selected reaction monitoring , biochemistry , catalysis
In this study, an analytical method was developed for quantification of residues of the anthelmintic drug phenothiazine (PTZ) in pork muscle using liquid chromatography–tandem mass spectrometry. Muscles were extracted using 0.2% formic acid and 10 m m ammonium formate in acetonitrile, defatted and purified using n ‐hexane. The drug was well separated on a Waters XBridge™ C 18 analytical column using a binary solvent system consisting of 0.2% formic acid and 10 m m ammonium formate in ultrapure water (A) and acetonitrile (B). Good linearity was achieved over a six‐point concentration range in matrix‐matched calibration with determination coefficient =0.9846. Fortified pork muscle having concentrations equivalent to and double the limit of quantification (1 ng/g) yielded recovery ranges between 100.82 and 104.03% and relative standard deviations <12%. Samples ( n = 5) collected from large markets located in Seoul City tested negative for PTZ residue. In conclusion, 0.2% formic acid and ammonium formate in acetonitrile can effectively extract PTZ from pork muscle without solid‐phase extraction, a step normally required for cleanup before analysis and the validated method can be used for routine analysis to ensure the quality of animal products.