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Validated high‐performance liquid chromatography method for the determination of the inhibitor of cancer cell invasion ( E )‐ N ‐benzyl‐6‐[2‐(3, 4‐dihydroxy benzylidene)hydrazinyl]‐ N ‐methylpyridine‐3‐sulfonamide in rat plasma and its application to pharmacokinetic study
Author(s) -
Kim Tae Kon,
Choi Hyun Kyung
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3875
Subject(s) - chemistry , chromatography , ammonium acetate , high performance liquid chromatography , detection limit , sulfonamide , pharmacokinetics , acetonitrile , ammonium , oral administration , pharmacology , stereochemistry , medicine , organic chemistry
In this study, a reliable method for the quantitation of ( E )‐ N ‐benzyl‐6‐[2‐(3, 4‐dihydroxy benzylidene)hydrazinyl]‐ N ‐methylpyridine‐3‐sulfonamide (JW‐55) in rat plasma was developed and validated using high‐performance liquid chromatography. Plasma samples were deproteinized; sildenafil was used as an internal standard. Chromatographic separation was achieved using a reversed‐phase C 18 column. The mobile phase, 0.02 m ammonium acetate buffer:acetonitrile (48:52, v /v), was run at a flow rate of 1.0 mL/min at room temperature, and the column eluent was monitored using an ultraviolet detector at 280 nm. The retention times of JW‐55 and sildenafil were ~5.9 and 7.7 min, respectively. The detection limit of JW‐55 in rat plasma was 0.03 μg/mL. Pharmacokinetic parameters of JW‐55 were evaluated after intravenous and oral administration of JW‐55 (10 mg/kg) in rats. After oral administration, the F value was approximately 73.7%.