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The disappearance rate and risk assessment of thiacloprid residues in Asian pear using liquid chromatography confirmed with tandem mass spectrometry
Author(s) -
Kabir Md. Humayun,
Abd ElAty A.M.,
Rahman Md. Musfiqur,
Kim SungWoo,
Choi JeongHeui,
Lee YoungJun,
Truong Lieu T.B.,
Lee KangBong,
Kim MiRa,
Shin HoChul,
Shim JaeHan
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3861
Subject(s) - chemistry , chromatography , residue (chemistry) , thiacloprid , detection limit , calibration curve , solid phase extraction , liquid chromatography–mass spectrometry , analyte , mass spectrometry , pesticide , biochemistry , imidacloprid , thiamethoxam , agronomy , biology
This study was undertaken to quantify the residue levels and propose the dissipation kinetics of thiacloprid formulated as suspension concentrate in field‐incurred Asian pears grown under two different open‐field conditions. Samples were extracted with 20% distilled water in acetonitrile; partitioned with brine water and dichloromethane; and purified with a Florisil solid phase extraction cartridge. The analyte was identified with an LC ultraviolet detector, and field‐incurred samples were confirmed using LC–MS/MS. The calibration curve was linear over the range 0.05–5.0 mg/L with a satisfactory coefficient of determination ( R 2 = 0.9994). The limits of detection and limits of quantification (LOQ) were 0.003 and 0.01 mg/kg, respectively. The recovery rate fortified to blank samples at LOQ, 10× LOQ, and the maximum residue limit (MRL) were between 73.7 and 86.2% with relative standard deviation ≤9.0%. The residual concentrations at both sites were considerably lower than the MRL (0.7 mg/kg) set by the Korean Ministry of Food Drug Safety, with biological half‐lives of 5.0 and 7.4 days, for sites 1 and 2, respectively. From the pre‐harvest residue limit curve, it was predicted that if the residues were <1.13 or 1.40 mg/kg 10 days before harvest, the residue level would be lower than the MRL during harvest. Risk assessment on day 0 showed an acceptable daily intake (%) of 13.0% and 11.0% for sites 1 and site 2, respectively, which indicates that the residual amounts are not hazardous to the Korean population.