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Intravenous administration of 2,7,8‐trimethyl‐2‐( β ‐carboxyethyl)‐6‐hydroxy chroman ( γ ‐CEHC) to rats and determination of its plasma concentration and urinary sodium excretion
Author(s) -
Tanabe Maiko,
Fukushima Takeshi,
Usui Noriko,
Aoyama Nozomi,
Tsunoda Makoto,
Imai Kazuhiro
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.385
Subject(s) - chemistry , chromatography , excretion , sodium , urine , urinary system , plasma , plasma concentration , pharmacology , endocrinology , biochemistry , organic chemistry , medicine , physics , quantum mechanics
A natriuretic hormone, 2,7,8‐trimethyl‐2‐( β ‐carboxyethyl)‐6‐hydroxy chroman ( γ ‐CEHC) was administered intravenously to male Sprague–Dawley rats and the plasma concentration of γ ‐CEHC along with urinary sodium (Na + ) excretion was investigated. The plasma γ ‐CEHC concentrations were uorimetrically determined by a column‐switching HPLC method consisting of both phenyl and octadecyl silica columns, following a pre‐column uorescence derivatization with a uorescence reagent, 4‐ N , N ‐dimethylaminosulfonyl‐7‐piperazino‐2,1,3‐benzoxadiazole (DBD‐PZ). In rats fed with a high‐NaCl (8.0%) diet, plasma γ ‐CEHC concentrations rapidly decreased by 20% in 15–45 min after the adminstration of γ ‐CEHC, while Na + excretion gradually increased with time. Considering these results, the Na + excretion effect appeared not to be associated with plasma γ ‐CEHC concentration. In addition, attempts were made to examine a main urinary metabolite of γ ‐CEHC, a large amount of 6‐ O ‐sulfated γ ‐CEHC found to be present in the urine using an HPLC‐tandem mass spectrometry. Thus, it is plausible that γ ‐CEHC was easily metabolized to 6‐ O ‐sulfated metabolite and excreted into urine in rats. Copyright © 2004 John Wiley & Sons, Ltd.