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Pharmacokinetic study of ardisiacrispin A in rat plasma after intravenous administration by UPLC–MS/MS
Author(s) -
Fang Bingmu,
Bao Shihui,
Wang Shuanghu,
Chen Minle,
Chen Bingbao,
Su Ke,
Wen Congcong,
Zhou Yunfang,
Wang Xianqin,
Jin Yuepeng
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3826
Subject(s) - chemistry , chromatography , protein precipitation , formic acid , electrospray ionization , selected reaction monitoring , pharmacokinetics , acetonitrile , high performance liquid chromatography , calibration curve , mass spectrometry , methanol , tandem mass spectrometry , detection limit , pharmacology , medicine , organic chemistry
In this work, a sensitive and selective UPLC‐MS/MS method for determination of ardisiacrispin A in rat plasma was developed. Cyasterone used as an internal standard (IS) and protein precipitation by acetonitrile–methanol (9:1, v /v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C 18 column (2.1 × 100 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m / z 1083.5 → 407.1 for ardisiacrispin A and m / z 521.3 → 485.2 for IS. Calibration plots were linear throughout the range 5–2000 ng/mL for ardisiacrispin A in rat plasma. Mean recoveries of ardisiacrispin A in rat plasma ranged from 80.4 to 92.6%. The values of RSD of intra‐ and inter‐day precision were both <11%. The accuracy of the method was between 97.3 and 105.6%. The method was successfully applied to pharmacokinetic study of ardisiacrispin A after intravenous administration in rats.