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A rapid and simple method for determination of halothane, isoflurane and sevoflurane in blood using gas chromatography
Author(s) -
Atherley Richard J.,
Antognini Joseph F.
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.382
Subject(s) - chemistry , chloroform , halothane , chromatography , anesthetic , solvent , methylene , volatile anesthetic , gas chromatography , chloride , anesthesia , organic chemistry , medicine , isoflurane
We have developed a technique to determine the concentration of volatile anesthetics (halothane, isourane and sevourane) in blood that is a modication of a method used for volatile anesthetics in Krebs solution. Methylene chloride was the internal standard and chloroform was used to extract the volatile anesthetic from blood. The congealed blood proteins were separated from the chloroform solvent (containing anesthetic) using a two‐compartment vial that ltered out the proteinaceous material during centrifuging. Recovery averaged 102%. Linearity was excellent ( r = 0.992–0.999) in the 50–600, 50–300 and 50–300 µg/mL range for halothane, isourane and sevourane, respectively. Intra‐day and inter‐day precisions were likewise excellent, with relative standard deviations <5.3 and <7.1%, respectively. Accuracy ranged from 0.8 to 9.5% of the estimated theoretical value. Extracted anesthetic in chloroform solvent was stable over 4–5 days, with <3% variability. The time from obtaining the blood sample to determination of the concentration from the chromatographic peak was 15 min or less. Copyright © 2004 John Wiley & Sons, Ltd.