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A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats
Author(s) -
Abdelkawy Khaled S.,
Balyshev Mark E.,
Elbarbry Fawzy
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3819
Subject(s) - chromatography , chemistry , high performance liquid chromatography , pharmacokinetics , protein precipitation , acetonitrile , relative standard deviation , accuracy and precision , detection limit , pharmacology , medicine , statistics , mathematics
A simple, accurate, and reproducible high‐performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20 A HPLC system equipped with a Supelcosil LC‐18 T C 18 column and an isocratic mobile phase consisting of 0.3% trichloroacetic acid in water and acetonitrile HPLC‐grade (50:50, v /v) run at a flow rate of 0.9 mL/min for 13 min. The UV detection wavelength was set at 254 nm. The method exhibited good linearity ( R 2 > 0.994) over the assayed concentration range (0.10–25 μg/mL) and demonstrated good intra‐day and inter‐day precision and accuracy (relative standard deviations and the deviation from predicted values were <20%). This method was also successfully applied for studying the pharmacokinetics of QR in rats following a single oral dose of QR to evaluate its pharmacokinetic parameters in rats.