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Application of Cookson‐type reagents for biomedical HPLC and LC/MS analyses: a brief overview
Author(s) -
Higashi Tatsuya,
Shimada Kazutake
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3808
Subject(s) - chemistry , reagent , adduct , derivatization , chromatography , mass spectrometry , high performance liquid chromatography , moiety , tandem mass spectrometry , conjugated system , fluorophore , fluorescence , organic chemistry , physics , quantum mechanics , polymer
Abstract High‐performance liquid chromatography (HPLC), LC/mass spectrometry (MS) and LC/tandem mass spectrometry (MS/MS) have been widely used for biomedical analyses, in which chemical derivatization is one of the most important methods to increase the sensitivity and selectivity. A Cookson‐type reagent [4‐substituted‐1,2,4‐triazoline‐3,5‐dione (4‐substituted‐TAD)] reacts with the compound bearing a conjugated diene, such as the vitamin D compound, to quantitatively form the stable Diels–Alder adduct. The reagent with a chromophore or fluorophore at the 4‐position of TAD yields a highly responsive adduct for the UV or fluorescence detection, respectively. The Diels–Alder adduct with a Cookson‐type reagent having a permanently charged, proton‐affinitive or electron‐affinitive moiety is sensitively detected by a specific MS analyzer. This paper is a brief overview of the applications of the reagents for biomedical analyses mainly using HPLC or LC/MS(/MS).