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Development and validation of a quantification method for oleanolic acid and hederagenin in rat plasma: application to the pharmacokinetic study
Author(s) -
Zhang Heng,
Jing Fanbo,
Zhang Zonglin
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3801
Subject(s) - hederagenin , oleanolic acid , chromatography , chemistry , pharmacokinetics , extraction (chemistry) , calibration curve , analyte , high performance liquid chromatography , detection limit , pharmacology , medicine , alternative medicine , pathology
A rapid and sensitive liquid chromatography tandem mass spectrometry (LC–MS/MS) was developed and validated for simultaneous quantification of oleanolic acid and hederagenin in rat plasma. After the two analytes were extracted with liquid–liquid extraction, chromatographic separation was performed on a C 18 column with acetonitrile and water (85:15, v /v) as mobile phase at a flow rate of 0.4 mL/min. Calibration curves exhibited good linearity ( r > 0.995) over the ranges of 0.41–82.0 ng/mL for oleanolic acid and 0.32–64.0 ng/mL for hederagenin, respectively. The lower limit of quantifications (LLOQs) in plasma were 0.41 ng/mL for oleanolic acid and 0.32 ng/mL for hederagenin. The established LLOQs were within the concentration needed for the assay in plasma, which met the requirements to evaluate their pharmacokinetics of oleanolic acid and hederagenin. This developed assay was successfully applied in the pharmacokinetic study of oleanolic acid and hederagenin in rats after oral administration of Rhizoma Clematidis extract.