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Simultaneous determination of ginsenoside Rg 1 , Re and notoginsenoside R 1 in human plasma by LC‐MS/MS and its application in a pharmacokinetic study in Chinese volunteers
Author(s) -
Zhang Xiqian,
Ma Rujun,
Liu Xiaojuan,
Jiang Xuehua,
Wang Ling
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3766
Subject(s) - chemistry , chromatography , ammonium formate , ginsenoside , pharmacokinetics , ginsenoside rg1 , calibration curve , selected reaction monitoring , liquid chromatography–mass spectrometry , tandem mass spectrometry , detection limit , accuracy and precision , ammonium acetate , mass spectrometry , high performance liquid chromatography , ginseng , pharmacology , medicine , statistics , alternative medicine , mathematics , pathology
A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method has been developed and validated for simultaneous quantification of ginsenosides Rg 1 , Re and notoginsenoside R 1 in human plasma. Chromatography was performed on Capcell Pak C 18 MG II column using a binary gradient using mobile phase A (5 m m ammonium formate solution) and B (methanol, containing 5 m m ammonium formate) at a flow rate of 0.3 mL/min. The entire chromatographic run time was 3.2 min. Quantification was achieved using multiple reaction monitoring in positive mode using API 3000. This method was validated in terms of specificity, linearity, precision, accuracy, matrix effect and stability. The calibration curves were linear in the concentration range of 0.020–5.00 ng/mL for ginsenosides Rg 1 , Re and notoginsenoside R 1 . The lower limit of quantification (LLOQ) of this method was 0.020 ng/mL. The intra‐run and inter‐run precision values were within 12.31% for ginsenoside Rg 1 , 14.13% for ginsenoside Re and 11.46% for notoginsenoside R 1 at their LLOQ levels. The samples were stable under all tested conditions. This method was successfully applied to study the pharmacokinetics of ginsenoside Rg 1 and notoginsenoside R 1 in 24 healthy volunteers following oral administration of 200 mg Sanqi Tongshu Enteric‐Pellets Capsule.