Premium
Quantitative determination of saroglitazar, a predominantly PPAR alpha agonist, in human plasma by a LC–MS/MS method utilizing electrospray ionization in a positive mode
Author(s) -
Ghoghari Ashok,
Dash Ranjeet,
Bhatt Chandrakant,
Singh Kanchan,
Jha Anil,
Patel Harilal,
Gupta Rahul,
Kansagra Kevinkumar,
Srinivas Nuggehally R.
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3760
Subject(s) - chemistry , chromatography , ammonium acetate , extraction (chemistry) , electrospray ionization , mass spectrometry , high performance liquid chromatography
A sensitive LC–MS/MS method was developed and validated for quantitation of saroglitazar using turboion spray interface with positive ion mode. A liquid–liquid extraction, with a mixture of dichloromethane and diethyl ether, was employed for the extraction of saroglitazar and glimepiride (IS) from human plasma. The chromatographic separation was achieved using an ACE‐5, C 18 (4.6 × 100 mm) column with a gradient mobile phase comprising acetonitrile and ammonium acetate buffer with trifluoracetic acid in purified water. Both analytes were separated within 10 min with retention times of 4.52 and 2.57 min for saroglitazar and IS, respectively. Saroglitazar quantitation was achieved by the summation of two MRM transition pairs ( m / z 440.2 to m / z 366.0 and m / z 440.2 to m / z 183.1), while that of IS was achieved using transition pair m / z 491.3 to m / z 352.0. The calibration standards of saroglitazar showed linearity from 0.2 to 500 ng/mL, with a lower limit of quantitation of 0.2 ng/mL. The biases for inter‐ and intra‐batch assays were −7.51–1.15% and −11.21 to −3.25%, respectively, while the corresponding precisions were 5.04–8.06% and 1.53–7.68%, respectively. The developed method was used to monitor the plasma concentrations of saroglitazar in clinical samples.