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Establishment of a ternary network system for evaluating the antioxidant fraction of Danhong injection
Author(s) -
Wang Yan,
Jiang Zhenzuo,
Yang Fan,
Chai Xin,
Zhu Yan,
Zhao Xiaoya,
Jiang Miaomiao,
Yang Jing,
Zhao Buchang,
Qian Ke,
Wang Yuefei
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3739
Subject(s) - chemistry , ternary operation , fraction (chemistry) , antioxidant , chromatography , organic chemistry , computer science , programming language
Oxidative stress plays a crucial role in numerous cardiovascular diseases. As an effective therapy, Danhong injection (DHI) is considered to act through an antioxidant mechanism for the treatment of cardiovascular disease. In our study, we focused on the potential contribution of the antioxidant capacity of DHI fractions (Frs) and established an innovative screening method based on a 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity assay. A ternary network evaluation system, which was constructed based on the radical scavenging activity, the area under the activity–concentration curve and the solid content of the fractions, was implemented to select the fractions that posed the greatest antioxidant effect. As a result, Frs 5–7 and Frs 17–19 were shown to exhibit superior antioxidant activity according to the regression area of the ternary network, which was >0.5. Furthermore, the active fractions were characterized by ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry combined with nuclear magnetic resonance. This study provided an effective method for the comprehensive evaluation of the antioxidant effect of DHI fractions. Copyright © 2016 John Wiley & Sons, Ltd.