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Predicting human intestinal absorption in the presence of bile salt with micellar liquid chromatography
Author(s) -
Waters Laura J.,
Shokry Dina S.,
Parkes Gareth M.B.
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3731
Subject(s) - chemistry , bioavailability , pulmonary surfactant , micellar liquid chromatography , chromatography , absorption (acoustics) , salt (chemistry) , in vivo , micelle , aqueous solution , organic chemistry , biochemistry , pharmacology , medicine , physics , microbiology and biotechnology , acoustics , biology
Understanding intestinal absorption for pharmaceutical compounds is vital to estimate the bioavailability and therefore the in vivo potential of a drug. This study considers the application of micellar liquid chromatography (MLC) to predict passive intestinal absorption with a selection of model compounds. MLC is already known to aid prediction of absorption using simple surfactant systems; however, with this study the focus was on the presence of a more complex, bile salt surfactant, as would be encountered in the in vivo environment. As a result, MLC using a specific bile salt has been confirmed as an ideal in vitro system to predict the intestinal permeability for a wide range of drugs, through the development of a quantitative partition–absorption relationship. MLC offers many benefits including environmental, economic, time‐saving and ethical advantages compared with the traditional techniques employed to obtain passive intestinal absorption values. Copyright © 2016 John Wiley & Sons, Ltd.