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Simultaneous determination of some active ingredients in anti‐viral preparations of traditional Chinese medicine by micellar electrokinetic chromatography
Author(s) -
Chen Xiaofeng,
Zhang Jiyou,
Xue Chunxia,
Chen Xingguo,
Hu Zhide
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.373
Subject(s) - chemistry , chromatography , micellar electrokinetic chromatography , borax , analyte , electrokinetic phenomena , chlorogenic acid , linear regression , caffeic acid , linear relationship , detection limit , raw material , biochemistry , statistics , mathematics , organic chemistry , machine learning , computer science , antioxidant
A simple and rapid micellar electrokinetic chromatography method was developed for the simultaneous determination of quercetin, gentiopicrin, forsythin, chlorogenic acid and caffeic acid in anti‐viral preparations of traditional Chinese medicine (apTCM). In this method, the effects of buffer pH, concentration of the borax and SDS, organic modiers, applied voltage and temperature on the separation were tested and discussed. The results showed that the ve analytes could be well separated within 11 min under conditions of 40 m m borax (pH 9.65) containing 20 m m SDS, 20 kV, at 25°C. In the tested concentration range, regression equations revealed good linear relationships (correlation coefcients 0.9920–0.9991) between the peak areas and corresponding concentrations. In addition, a multiple linear regression QSPR model was constructed to predict the migration times of the analytes and the results were satisfactory. The method was validated by analysis of the ve compounds in three representative apTCM samples with recoveries ranging from 89.2 to 106.6%. Copyright © 2004 John Wiley & Sons, Ltd.