Development and validation of a sensitive LC–MS/MS assay for quantification of bruceine D in rat plasma | Zendy

Zhang Yining | Zendy; Zhen Yong | Zendy; Zhang Ying | Zendy; Liu Shouyue | Zendy

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Development and validation of a sensitive LC–MS/MS assay for quantification of bruceine D in rat plasma

Author(s) -

Zhang Yining,

Zhen Yong,

Zhang Ying,

Liu Shouyue

Publication year - 2016

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.3724

Subject(s) - chemistry , chromatography

A sensitive LC–MS/MS method for the determination of bruceine D in rat plasma was developed. The analyte and IS were separated on a Luna C 18 column (2.1 × 50 mm, 1.7 μm) using a mobile phase of acetonitrile and 0.1% formic acid in water (40:60, v/v) at a flow rate of 0.25 mL/min. The selected reaction monitoring mode was chosen to monitor the precursor‐to‐product ion transitions of m/z 409.2 → 373.2 for bruceine D and m/z 469.2 → 229.3 for IS using a negative ESI mode. The method was validated over a concentration range of 0.5–2000 ng/mL for bruceine D. Total chromatography time for each run was 3.5 min. The method was successfully applied to a pharmacokinetic study of bruceine D in rats. Copyright © 2016 John Wiley & Sons, Ltd.

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