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Simultaneous estimation of levodopa, carbidopa and 3‐oxymethyldopa in rat plasma using HPLC‐ECD
Author(s) -
Raut Prashant P.,
Charde Shrikant Y.,
Bishnoi Pradeep
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3713
Subject(s) - chemistry , chromatography , repeatability , carbidopa , analyte , pharmacokinetics , high performance liquid chromatography , accuracy and precision , pellets , phosphate buffered saline , methanol , plasma , levodopa , relative standard deviation , detection limit , pharmacology , medicine , statistics , oceanography , mathematics , disease , organic chemistry , physics , quantum mechanics , parkinson's disease , geology , pathology
The aim of study was to develop a suitable analytical method for simultaneous estimation of levodopa, carbidopa and 3‐ O ‐methyl dopa in rat plasma. Chromatographic separation of plasma samples was achieved using a reverse‐phase C 18 column. The mobile phase used consisted of a mixture of methanol and phosphate buffer (10 m M , pH 3.50) in the ratio of 90:10 v/v. All analytes were estimated by electrochemical detection at +800 mV. The developed method has been validated as per the standard guidelines. Precision study results were found to be satisfactory, with percentage relative standard deviation for repeatability and intermediate precision <3.96 and 6.56%, respectively, for all analytes detected in rat plasma. The developed method in rat plasma was found to be simple, rapid, accurate, precise and specific. The proposed method has been successfully applied for analysis of rat plasma samples obtained during an oral pharmacokinetic study of sustained release pellets of levodopa and carbidopa in rats. Copyright © 2016 John Wiley & Sons, Ltd.