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Capillary electrophoresis of catecholamines with laser‐induced fluorescence intensified charge‐coupled device detection
Author(s) -
Xiong Shaoxiang,
Han Huiwan,
Zhao Rui,
Chen Yi,
Liu Guoquan
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.37
Subject(s) - chemistry , capillary electrophoresis , chromatography , laser induced fluorescence , derivatization , fluorescence , fluorescein isothiocyanate , capillary action , analytical chemistry (journal) , electrophoresis , detection limit , mass spectrometry , materials science , physics , quantum mechanics , composite material
A capillary electrophoresis–laser‐induced fluorescence–intensified charge‐coupled device system was used for the separation and determination of catecholamines. Optimization of derivatization and separation conditions was investigated in order to reach a high separation efficiency and sensitivity. All fluorecein isothiocyanate derivatives of catecholamines were satisfactorily separated within 12 min. The detection limits were in attomole ranges. This method allows determination of catecholamines with high separation efficiencies and sensitivity. Copyright © 2001 John Wiley & Sons, Ltd. Abbreviations used: CBQCA 3‐(4‐carboxybenzoyl)‐2‐quinoline carboxaldehydeCCD charge‐coupled deviceDA dopamine, E, epinephrineFITC fluorescein isothiocyanateS‐HT serotoni Lif, laser‐induced fluorescenceNE noreginephrine.