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Hair analysis of nicotine and cotinine for evaluating tobacco smoke exposure by liquid chromatography–mass spectrometry
Author(s) -
Chetiyanukornkul Thaneeya,
Toriba Akira,
Kizu Ryoichi,
Kimura Kazuko,
Hayakawa Kazuichi
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.369
Subject(s) - chemistry , chromatography , cotinine , nicotine , dichloromethane , mass spectrometry , methanol , solvent , organic chemistry , neuroscience , biology
A simple liquid chromatography–electrospray ionization mass spectrometry (LC‐ESI‐MS) method for the determination of nicotine and cotinine in human hair was established. In the procedure, a hair sample (10 mg) was washed with dichloromethane and digested in 2.5 m sodium hydroxide. The digest was extracted with dichloromethane and then 25 m m hydrochloric acid in methanol was added to the extract, to prevent loss of analytes. The solution was evaporated and redissolved in the mobile phase, methanol/10 m m ammonium acetate (30/70, v/v). A 20 µL aliquot of redissolved solution was subjected to analysis. Nicotine and cotinine in human hair were quantied by using deuterated analytes as internal standards. The quantication limits were 8 µg/L for nicotine and 0.9 µg/L for cotinine. The proposed method was applied to measure the concentrations of nicotine and cotinine in hair of smokers and non‐smokers to evaluate their self‐reported smoking and exposure to environmental tobacco smoke. In both cases, the method provided good selectivity, accuracy and precision. Copyright © 2004 John Wiley & Sons, Ltd.