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Sensitive method for the determination of rocilinostat in small volume mouse plasma by LC‐MS/MS and its application to a pharmacokinetic study in mice
Author(s) -
Gupta Manish,
Dixit Abhishek,
Devaraj V. C.,
Zainuddin Mohd,
Bhamidipati Ravi Kanth,
Hallur Mahanandeesha S.,
Dewang Purushottam,
Rajagopal Sridharan,
Rajagopal Sriram,
Mullangi Ramesh
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3665
Subject(s) - chemistry , chromatography , protein precipitation , formic acid , pharmacokinetics , acetonitrile , elution , high performance liquid chromatography , pharmacology , medicine
A highly sensitive, specific and rapid LC‐ESI‐MS/MS method has been developed and validated for the quantification of rocilinostat in small volume mouse plasma (20 μL) using vorinostat as an internal standard (IS) as per regulatory guidelines. Sample preparation was accomplished through a protein precipitation procedure with acetonitrile. Chromatography was achieved on Prodigy ODS‐2 column using a binary gradient using mobile phase A (0.2% formic acid in water) and B (acetonitrile) at a flow rate of 0.38 mL/min. The total chromatographic run time was 4.1 min and the elution of rocilinostat and IS occurred at ~3.2 and 2.9 min, respectively. A linear response function was established in the concentration range of 0.28–1193 ng/mL in mouse plasma. The intra‐ and inter‐day accuracy and precisions were in the ranges of 3.12–8.93 and 6.41–11.6%, respectively. This novel method has been applied to a pharmacokinetic study in mice. Copyright © 2016 John Wiley & Sons, Ltd.