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Analysis of mandipropamid residual levels through systematic method optimization against the matrix complexity of sesame leaves using HPLC/UVD
Author(s) -
Farha Waziha,
Rahman Md. Musfiqur,
Abd ElAty A. M.,
Kim SungWoo,
Jung DaI,
Im So Jeong,
Choi JeongHeui,
Kabir Md. Humayun,
Lee KangBong,
Shin HoChul,
Shim JaeHan
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3638
Subject(s) - chromatography , chemistry , detection limit , solid phase extraction , calibration curve , residue (chemistry) , cartridge , high performance liquid chromatography , matrix (chemical analysis) , extraction (chemistry) , sorbent , analytical chemistry (journal) , materials science , biochemistry , organic chemistry , adsorption , metallurgy
An analytical method was developed to detect mandipropamid residues in sesame leaves using high‐performance liquid chromatography–ultraviolet detection. Samples were extracted with acetonitrile and were prepurified using a solid‐phase extraction (SPE) cartridge with an additional dispersive‐SPE (d‐SPE) sorbent application. The method was validated using an external calibration curve prepared using pure solvent. The linearity was excellent with determination coefficient = 1. The limits of detection and quantification were 0.003 and 0.01 mg/kg, respectively. Recoveries at three spiking levels – 0.1, 0.5, and 1.0 mg/kg – were in the range 80.3–90.7% with relative standard deviations <2%. This method was applied to field‐treated samples collected from two different areas, Gwangju and Muan, in the Republic of Korea and the half‐lives were similar, 5.10 and 5.41 days, respectively. The pre‐harvest residue limit was also predicted for both sites. The proposed method is sensitive and able to quantify trace amounts of mandipropamid in leafy vegetables. The combination of SPE and d‐SPE effectively removed the matrix components in sesame leaves. Copyright © 2015 John Wiley & Sons, Ltd.