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Metabolic routes along digestive system of licorice: multicomponent sequential metabolism method in rat
Author(s) -
Zhang Lei,
Zhao Haiyu,
Liu Yang,
Dong Honghuan,
Lv Beiran,
Fang Min,
Zhao Huihui
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3626
Subject(s) - glycyrrhiza uralensis , chemistry , metabolism , metabolic pathway , chromatography , blood sampling , drug metabolism , in vivo , high performance liquid chromatography , pharmacology , glycyrrhiza , incubation , biochemistry , biology , microbiology and biotechnology , medicine , alternative medicine , pathology
This study was conducted to establish the multicomponent sequential metabolism (MSM) method based on comparative analysis along the digestive system following oral administration of licorice ( Glycyrrhiza uralensis Fisch., leguminosae), a traditional Chinese medicine widely used for harmonizing other ingredients in a formulae. The licorice water extract (LWE) dissolved in Krebs–Ringer buffer solution (1 g/mL) was used to carry out the experiments and the comparative analysis was performed using HPLC and LC‐MS/MS methods. In vitro incubation, in situ closed‐loop and in vivo blood sampling were used to measure the LWE metabolic profile along the digestive system. The incubation experiment showed that the LWE was basically stable in digestive juice. A comparative analysis presented the metabolic profile of each prototype and its corresponding metabolites then. Liver was the major metabolic organ for LWE, and the metabolism by the intestinal flora and gut wall was also an important part of the process. The MSM method was practical and could be a potential method to describe the metabolic routes of multiple components before absorption into the systemic blood stream. Copyright © 2015 John Wiley & Sons, Ltd.