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Quantification of complanatoside A in rat plasma using LC‐MS/MS and its application to a pharmacokinetic study
Author(s) -
Li Nan,
Liu Yue,
Cao Yuchen,
Wei Zhouxia,
Pang Li,
Wang Jianmeng
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3624
Subject(s) - chromatography , chemistry , protein precipitation , electrospray ionization , extraction (chemistry) , pharmacokinetics , matrix (chemical analysis) , mass spectrometry , pharmacology , medicine
Complanatoside A is a flavonol glycoside isolated from Astragalus complanatus , and currently it is used as a quality control index for A. complanatus in the 2010 edition of the Chinese Pharmacopoeia . For the first time, a simple and sensitive LC‐MS/MS method was developed for the determination of complanatoside A in rat plasma over the range of 2.3–575 ng/mL. Complanatoside A was extracted from plasma by a protein precipitation procedure, separated by LC and detected by MS/MS in positive electrospray ionization mode. The method was validated for selectivity, carryover, sensitivity, linearity, extraction recovery, matrix effect, accuracy, precision and stability studies. The lower limit of quantification was established at 2.3 ng/mL. Intra‐ and inter‐day precisions (LLOQ, low‐QC, med‐QC and high‐QC) were <7.9%, and accuracies were between 94.0 and 105.1%. Matrix effect was acceptable (97.9–103.0%) and extraction recovery was reproducible (88.5–94.4%). Complanatoside A was stable in the investigated conditions. The method was applied to the pharmacokinetics of complanatoside A in rats. Copyright © 2015 John Wiley & Sons, Ltd.