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LC‐MS based metabolomics identification of novel biomarkers of tobacco smoke‐induced chronic bronchitis
Author(s) -
Ren Xiaolei,
Zhang Jiayu,
Fu Xiaorui,
Ma Shuangshuang,
Wang Chunguo,
Wang Juan,
Tian Simin,
Liu Siqi,
Zhao Baosheng,
Wang Xueyong
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3620
Subject(s) - chemistry , metabolomics , chromatography , tobacco smoke , chronic bronchitis , cigarette smoke , identification (biology) , organic chemistry , botany , medicine , environmental health , biology
Tobacco smoke (TS) is a major causative agent to lead to chronic bronchitis (CB). However the mechanisms of CB induced by TS are unclear. In this report, rats were exposed to different concentrations of TS and the metabolic features of CB were characterized by using a nontargeted metabolic profiling method based on liquid chromatography‐mass spectrometry (LC‐MS) to detect the altered metabolic patterns in serum from CB rats and investigate the mechanisms of CB. 11 potential biomarkers were identified in serum of rats. Among them, the levels of lysophosphatidylethanolamine (18:1), lysophosphatidic acid (18:1), lysophosphatidylethanolamine (18:0), lysophosphatidylethanolamine (16:0), lysophosphatidylethanolamine (20:4), docosahexaenoic acid, 5‐hydroxyindoleacetic acid and 5′‐carboxy‐ γ ‐tocopherol were higher in TS group compared to control group. Conversely, the levels of 4‐imidazolone‐5‐propionic acid, 12‐hydroxyeicosatetraenoic acid and uridine were lower in TS group. The results indicated that the mechanism of CB was related to amino acid metabolism and lipid metabolism, particularly lipid metabolism. In addition, lysophosphatidylethanolamines were proved to be important mediators, which could be used as biomarkers to diagnose CB. These results also suggested that metabolomics was suitable for diagnosing CB and elucidating the possible metabolic pathways of TS‐induced CB. Copyright © 2015 John Wiley & Sons, Ltd.