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Microemulsion electrokinetic chromatography with laser‐induced fluorescence detection: as tested with amino acid derivatives
Author(s) -
Jianping Xie,
Jiyou Zhang,
Huanxiang Liu,
Jiaqin Liu,
Jianniao Tian,
Xingguo Chen,
Zhide Hu
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.362
Subject(s) - chemistry , microemulsion , chromatography , electrokinetic phenomena , micellar electrokinetic chromatography , electrophoresis , biochemistry , pulmonary surfactant
Over a decade ago, microemulsion electrokinetic chromatography was introduced as a novel mode of capillary electrophoresis. However, there has not been publication on the combination of microemulsion electrokinetic chromatography with laser‐induced uorescence detection. In this paper, a preliminary method using microemulsion eletrokinetic chromatography combined with laser‐induced uorescence detection and second derivative electrophoregram was established as a sensitive and selective assay for separation and determination of nine amino acids after derivatization with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1, 3‐diazol. The derivatization and separation conditions were optimized. In the investigated concentration ranges correlation coefcients were better than 0.995. The relative standard deviation ( n = 5) of the migration times and peak heights were 0.56–0.76 and 2.21–7.15%, respectively. The detection limits (S/N = 3) were at a neaomolar level (0.32–2.20 n m ). The method was applied for the analysis of compound amino acid injection and a Chinese traditional herbal medicine. The recoveries were 95.9–107.9%. Copyright © 2004 John Wiley & Sons, Ltd.