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A sensitive and selective LC‐MS/MS method for the quantitative determination of segetalin A from the plasma of rats
Author(s) -
Wang Jianmeng,
Zhang Nan,
Pang Li,
Xu Dahai,
Yan Weiwei,
Wu Jiang,
Chen Haiyan
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3602
Subject(s) - chemistry , chromatography , calibration curve , pharmacokinetics , formic acid , analyte , detection limit , selected reaction monitoring , quantitative analysis (chemistry) , high performance liquid chromatography , elution , mass spectrometry , tandem mass spectrometry , pharmacology , medicine
A sensitive and selective LC‐MS/MS method was developed and validated for the determination and pharmacokinetic investigation of segetalin A in rat plasma. Sample preparation was accomplished through a simple SPE procedure for the removal and preconcentration of the analyte and IS. Plasma samples were separated by HPLC on a Symmetry C 18 column using a mobile phase consisting of methanol and 0.1% formic acid in water (70:30, v/v) with isocratic elution. The quantification was performed using multiple reaction monitoring with the transitions m/z 610.3 → 511.2 for segetalin A and m/z 779.4 → 751.4 for IS, respectively. The calibration curve was linear over the range of 8.0–4000 ng/mL with a limit of quantitation (LOQ) of 8.0 ng/mL. This method was applied in a pharmacokinetic study of segetalin A in rats. For intravenous (i.v.) administration, the plasma concentrations of segetalin A decreased quickly ( t 1/2 z , 1.31 ± 0.341 h). For oral administration, the plasma concentrations of segetalin A increased to a peak value at 1.50 ± 0.577 h, followed by a gradual decrease to the LOQ in 12 h. The mean AUC values after i.v. and oral administration were 553 ± 105 and 1482 ± 110 ng h/mL, respectively. Copyright © 2015 John Wiley & Sons, Ltd.