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An efficient in vitro and in vivo HPLC method for hydnocarpin in nanomicelles formulation
Author(s) -
Feng Yingshu,
Sun Congyong,
OmariSiaw Emmanuel,
Zhu Yuan,
Tong Shanshan,
Wan Jinyi,
Yu Jiangnan,
Xu Ximing
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3566
Subject(s) - in vivo , chemistry , chromatography , high performance liquid chromatography , isoliquiritigenin , in vitro , pharmacokinetics , distribution (mathematics) , acetic acid , pharmacology , biochemistry , mathematics , medicine , mathematical analysis , microbiology and biotechnology , biology
Abstract For quantitative and other related bioactive studies of hydnocarpin, there is a need to establish an efficient, specific and sensitive analytical method ( in vitro and in vivo ). In this paper, an efficient HPLC method has been established and validated to analyze hydnocarpin in a nanomicelle formulation for the first time. Various chromatographic conditions for in vitro and in vivo determinations were investigated, with the application examined by pharmacokinetics and tissue distribution studies. The analysis was carried out using an HPLC system with a Waters symmetry C 18 column (4.6 × 150 mm, 5 µm) at 25°C with a detecting wavelength of 342 nm. Eluting at a rate of 1.0 mL/min, a 65% methanol and 35% acetic acid solution (0.1%) served as the mobile phase for the in vitro determinations while a 62% methanol and 38% acetic acid solution (0.1%) was used for in vivo analysis with isoliquiritigenin as internal standard. The established in vitro linearity range for hydnocarpin was 0.2–20 µg/mL ( R 2 = 0.9996), with the biological ( in vivo ) samples following the same trend. The accuracy of the method was >99% ( in vitro ) and 92.4–105.3% ( in vivo ). Also, the precision met the acceptance criterion. These results indicate that the established method exhibited high specificity, accuracy, linearity and precision. Additionally, this efficient HPLC method was applied to pharmacokinetics and tissue distribution studies. Copyright © 2015 John Wiley & Sons, Ltd.