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Determination of salvianolic acid C in rat plasma using liquid chromatography–mass spectrometry and its application to pharmacokinetic study
Author(s) -
Song Junke,
Zhang Wen,
Sun Jialin,
Zhang Xue,
Xu Xiaona,
Zhang Li,
Feng Zhangying,
Du Guanhua
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3558
Subject(s) - chemistry , chromatography , mass spectrometry , electrospray ionization , extraction (chemistry) , analyte , pharmacokinetics , selected reaction monitoring , triple quadrupole mass spectrometer , high performance liquid chromatography , electrospray , bioavailability , selected ion monitoring , quadrupole mass analyzer , ethyl acetate , liquid chromatography–mass spectrometry , tandem mass spectrometry , gas chromatography–mass spectrometry , medicine , bioinformatics , biology
A sensitive and reliable LC‐ESI‐MS method for the determination of salvianolic acid C in rat plasma has been developed and validated. Plasma samples were prepared by liquid–liquid extraction with ethyl acetate and separated on a Zorbax SB‐C 18 column (3.5 µm, 2.1 × 100 mm) at a flow rate of 0.3 mL/min using acetonitrile–water as mobile phase. The detection was carried out by a single quadrupole mass spectrometer with electrospray ionization source and selected ion monitoring mode. Linearity was obtained for salvianolic acid C ranging from 5 to 1000 ng/mL. The intra‐ and inter‐day precisions (RSD, %) didn't exceed 9.96%, and the accuracy (RE, %) were all within ±3.64%. The average recoveries of the analyte and internal standard were >89.13%. Salvianolic acid C was proved to be stable during all sample storage, preparation and analytic procedures. The validated method was successfully applied to pharmacokinetic study after oral and intravenous administration of salvianolic acid C to rats. The absolute oral bioavailability of salvianolic acid C was 0.29 ± 0.05%. This method was further applied to simultaneous determination of salvianolic acid A, salvianolic acid B and salvianolic acid C in rat plasma and showed good practicability. Copyright © 2015 John Wiley & Sons, Ltd.