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Determination of glucosamine and its derivatives released from photocrosslinked gelatin hydrogels using HPLC
Author(s) -
Suo Hairui,
Xu Kedi,
Zhang Hengyi,
Zheng Xiaoxiang
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3533
Subject(s) - chemistry , chromatography , glucosamine , high performance liquid chromatography , gelatin , self healing hydrogels , phosphate buffered saline , calibration curve , extraction (chemistry) , detection limit , acetonitrile , solid phase extraction , polymer chemistry , organic chemistry
A simple, accurate and validated reverse‐phase high‐performance liquid chromatography (HPLC)/UV method is developed for the determination of glucosamine hydrochloride (GlcN), N ‐acetyl‐glucosamine (NAG) and N ‐acryloyl‐glucosamine (AGA) released from photocrosslinked gelatin hydrogels. The HPLC separation was achieved on a Shimadzu InertSustain amino column (250 × 4.6 mm, 5 µm particle size) at room temperature using a mobile phase of acetonitrile–phosphate buffer (75:25, v/v, pH 6.0) at a flow rate of 1.0 mL/min and UV detection of 194 nm. The method was validated for specificity, linearity, limit of detection and quantification, accuracy, precision, extraction recovery and solution stability. The calibration curves were with excellent linearity, with correlation coefficients ( R 2 ) >0.999 for all three drugs. The intra‐ and inter‐day variation was <3.10% and the relative error was between −1.43 and 1.78%. The extraction recovery results ranged from 94.62 to 99.33%, demonstrating the absence of matrix effect. The sample and standard solutions were stable for more than 2 months. The method was successfully used for the analysis of released properties of drugs physically encapsulated and chemically crosslinked in the gelatin hydrogels. Copyright © 2015 John Wiley & Sons, Ltd.