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Simultaneous separation and sensitive detection of naringin and naringenin in nanoparticles by chromatographic method indicating stability and photodegradation kinetics
Author(s) -
Cordesi Leticia Malgarim,
Bromberger Nathany Genro,
Raffin Renata Platcheck,
Scherman Elfrides Eva
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3531
Subject(s) - naringin , chemistry , chromatography , photodegradation , naringenin , kinetics , high performance liquid chromatography , repeatability , nanoparticle , acetonitrile , organic chemistry , nanotechnology , flavonoid , catalysis , materials science , physics , photocatalysis , quantum mechanics , antioxidant
Abstract A simple, sensitive, precise and linear method by liquid chromatography was established for simultaneous determination and quantification of naringin and naringenin in polymeric nanoparticles. The method results in excellent separation in <11 min and with a peak purity of both flavonoids. The analyses were performed using a C 18 column (4.6 × 150 mm, 5 µm), at a 1 mL/min flow rate. The mobile phase consisted of a gradient of acetonitrile–water (pH 4.0; v/v) at a temperature of 25°C. The nanoparticles were prepared according to the method of interfacial deposition of a pre‐formed polymer. The method were validated in compliance with guidelines, and was found to be linear in the 1–40 µg/mL concentration range for both naringin and naringenin ( r > 0.99). Repeatability was determined at three concentration levels, obtaining an RSD (%) <0.9%, and the accuracy of the method was >98%. The photodegradation kinetics was determined for naringin; the coefficient that best represents degradation was of first order and naringenin presented a zero‐order kinetics. To our knowledge, a rapid and sensitive method for naringin and naringenin in polymeric nanoparticles has not been published elsewhere and this method is applicable to simultaneous evaluation of flavonoids. Copyright © 2015 John Wiley & Sons, Ltd.