Premium
Highly sensitive HPLC‐DAD method for the assay of gefitinib in patient plasma and cerebrospinal fluid: application to a blood‐brain barrier penetration study
Author(s) -
Fang Luo,
Song Yu,
Weng Xu,
Li Fanzhu,
Xu Yaping,
Lin Nengming
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3520
Subject(s) - chromatography , chemistry , cerebrospinal fluid , triethylamine , high performance liquid chromatography , penetration (warfare) , acetonitrile , elution , solid phase extraction , extraction (chemistry) , medicine , organic chemistry , pathology , operations research , engineering
The quantification of intracranial gefitinib (GEF) exposure is limited owing to the sensitivity of analytical equipment. Although mass spectrometry (MS) is the preferred method because of its high sensitivity, the equipment is not available in many laboratories, especially in developing Asian countries. In this paper, we developed a highly sensitive high performance liquid chromatography‐diode array detector (HPLC‐DAD) method for the assay of GEF in human cerebrospinal fluid (CSF) and plasma. GEF was extracted from CSF and plasma by solid‐phase extraction and liquid–liquid extraction, respectively. The chromatographic separation was performed on a C 18 column with gradient elution of 0.1% triethylamine solution and acetonitrile, then finally detected at 344 nm. This method was validated and proved to be highly sensitive with a lower limit of quantitation value of 0.11 ng/mL in CSF and 11 ng/mL in plasma. The blood–brain barrier penetration ratio of GEF ranged from 1.48 to 2.41%. This method provides a reliable MS‐independent solution for the quantitation of GEF in patients’ CSF and plasma. Copyright © 2015 John Wiley & Sons, Ltd.