Premium
Brain metabolomics in rats after administration of ketamine
Author(s) -
Wen Congcong,
Zhang Meiling,
Zhang Yuan,
Sun Fa,
Ma Jianshe,
Hu Lufeng,
Lin Guanyang,
Wang Xianqin
Publication year - 2016
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3518
Subject(s) - ketamine , metabolomics , chemistry , gas chromatography–mass spectrometry , chromatography , pharmacology , urea , uridine , partial least squares regression , mass spectrometry , biochemistry , anesthesia , rna , medicine , statistics , mathematics , gene
In this study, we developed a brain metabolomic method, based on gas chromatography–mass spectrometry (GC/MS), to evaluate the effect of ketamine on rats. Pattern recognition analysis, including both principal component analysis and partial least squares‐discriminate analysis revealed that ketamine induced metabolic perturbations. Compared with the control group, the levels of glycerol, uridine, cholesterol in rat brain of the ketamine group (50 mg/kg, 14 days) decreased, while the urea levels increased. Our results indicate that metabolomic methods based on GC/MS may be useful to elucidate ketamine abuse through the exploration of biomarkers. Copyright © 2015 John Wiley & Sons, Ltd.