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Simultaneous determination of ascaridole, p ‐cymene and α ‐terpinene in rat plasma after oral administration of Chenopodium ambrosioides L. by GC‐MS
Author(s) -
Hu Xiaoqian,
Chu Yang,
Ma Gang,
Li Wei,
Wang Xiangyang,
Mo Hongmei,
Yin Qihui,
Guo Jiahua,
Ma Xiaohui,
Zhou Shuiping
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3479
Subject(s) - chemistry , chromatography , detection limit , ethyl acetate
A sensitive and reliable GC‐MS method was developed and validated for the simultaneous determination of ascaridole, p ‐cymene and α ‐terpinene in rat plasma using naphthalene as internal standard. The plasma samples were extracted with ethyl acetate. Chromatographic separation was carried out on a HP‐5MS capillary analytical column (30 m × 0.25 mm, 0.25 µm) and detection was performed on a quadrupole mass spectrometer detector operated under selected ion monitoring mode. The method showed excellent linearity over the investigated concentration range ( r > 0.99) with the limit of quantitation down to 50, 10 and 5 ng/mL for ascaridole, p ‐cymene and α ‐terpinene, respectively. The intra‐day and inter‐day precisions (RSD) were <11.3%, and the accuracy was between 90.7 and 113.8%. The method was successfully applied to investigate the pharmacokinetics of Chenopodium ambrosioides L. following oral administration to rats. Copyright © 2015 John Wiley & Sons, Ltd.