An LC‐MS/MS method for determination of calceorioside B with cardiomyocyte protective activity in rat plasma and application to a pharmacokinetic study | Zendy

Yang Fan | Zendy; Song Yanqing | Zendy; Zhang Sixi | Zendy; Zhou Wei | Zendy

Premium

An LC‐MS/MS method for determination of calceorioside B with cardiomyocyte protective activity in rat plasma and application to a pharmacokinetic study

Author(s) -

Yang Fan,

Song Yanqing,

Zhang Sixi,

Zhou Wei

Publication year - 2015

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.3464

Subject(s) - chemistry , chromatography , ammonium acetate , formic acid , electrospray ionization , pharmacokinetics , selected reaction monitoring , mass spectrometry , methanol , tandem mass spectrometry , liquid chromatography–mass spectrometry , elution , high performance liquid chromatography , medicine , organic chemistry

A simple, sensitive and rapid liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed and validated for the determination of calceorioside B (CLB) in rat plasma. Detection was performed on a Thermo Scientific Hypersil Gold chromatography column using isocratic elution with a mobile phase of methanol–5 m m ammonium acetate–formic acid (70:30:0.1, v/v/v). Mass spectrometry was performed in selection reaction monitoring mode using a positive electrospray ionization interface. Good linearity was found for CLB in plasma in the linear range of 1.00–500 ng/mL ( r > 0.9960). The validated method was successfully applied to the pharmacokinetic study of CLB in rats. Copyright © 2015 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research