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An LC‐MS/MS method for determination of calceorioside B with cardiomyocyte protective activity in rat plasma and application to a pharmacokinetic study
Author(s) -
Yang Fan,
Song Yanqing,
Zhang Sixi,
Zhou Wei
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3464
Subject(s) - chemistry , chromatography , ammonium acetate , formic acid , electrospray ionization , pharmacokinetics , selected reaction monitoring , mass spectrometry , methanol , tandem mass spectrometry , liquid chromatography–mass spectrometry , elution , high performance liquid chromatography , medicine , organic chemistry
A simple, sensitive and rapid liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed and validated for the determination of calceorioside B (CLB) in rat plasma. Detection was performed on a Thermo Scientific Hypersil Gold chromatography column using isocratic elution with a mobile phase of methanol–5 m m ammonium acetate–formic acid (70:30:0.1, v/v/v). Mass spectrometry was performed in selection reaction monitoring mode using a positive electrospray ionization interface. Good linearity was found for CLB in plasma in the linear range of 1.00–500 ng/mL ( r > 0.9960). The validated method was successfully applied to the pharmacokinetic study of CLB in rats. Copyright © 2015 John Wiley & Sons, Ltd.