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Rapid separation of barbiturates and benzodiazepines by capillary electrochromatography with 3‐(1,8‐naphthalimido)propyl‐modified silyl silica gel
Author(s) -
Ohyama Kaname,
Wada Mitsuhiro,
Ohba Yoshihito,
Fujishita Osamu,
Nakashima Kenichiro,
Kuroda Naotaka
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.346
Subject(s) - capillary electrochromatography , chemistry , barbital , chromatography , oxazepam , secobarbital , electrochromatography , silica gel , nitrazepam , silylation , diazepam , capillary electrophoresis , benzodiazepine , pentobarbital , organic chemistry , pharmacology , medicine , biochemistry , receptor , catalysis
A capillary electrochromatographic (CEC) method was applied to the simultaneous separation of barbiturates (barbital, phenobarbital, secobarbital and thiopental) and benzodiazepines (nitrazepam, diazepam and triazolam). The separation was performed in a 75 µm i.d. capillary, packed with 3‐(1,8‐naphthalimido)propyl‐modied silyl silica gel (NAIP), studying the effects of buffer pH and mobile phase composition. Using an applied voltage of 20 kV and the short‐end injection method (9 cm capillary effective length), the mobile phase of 1.0 m m citrate buffer (pH 5.0) containing 45% methanol provided the baseline separation of seven toxic drugs in less than 9 min. In CEC with NAIP, the benzodiazepines were separated by the combination of hydrophobic and π – π interactions, whereas the separation of the barbiturates was based on the hydrophobic interaction. Copyright © 2004 John Wiley & Sons, Ltd.