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Development and validation of a high performance liquid chromatography method for determination of 6‐benzyl‐1‐benzyloxymethyl‐5‐iodouracil (W‐1), a novel non‐nucleoside reverse transcriptase inhibitor and its application to a pharmacokinetic study in rats
Author(s) -
Lu YingYuan,
Wang Xin,
Wang XiaoWei,
Liu JunYi,
Li Pu,
Ren Hong,
Lou YaQing,
Lu Chuang,
Zhang GuoLiang
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3457
Subject(s) - chemistry , chromatography , high performance liquid chromatography , calibration curve , detection limit , pharmacokinetics , extraction (chemistry) , acetonitrile , pharmacology , medicine
A sensitive and selective high‐performance liquid chromatographic (HPLC) method for determination of 6‐benzyl‐1‐benzyloxymethyl‐5‐iodouracil (W‐1), a novel non‐nucleoside reverse transcriptase inhibitor in rat plasma, was developed and validated. Chromatographic separation of W‐1 and megestrol acetate (internal standard) was achieved on a reversed‐phase C 18 column at 25°C. The mobile phase was consisted of acetonitrile–water (60:40, v/v) and pumped at a flow rate of 1.0 mL/min. The ultraviolet (UV) detector was set at the absorption wavelength of 284 nm. The calibration curve for W‐1 was linear over the concentration range of 0.01–8 µg/mL and the lower limit of quantification was 10 ng/mL. The intra‐ and inter‐day precision and accuracy were <8.9 and 5.3%, respectively. The extraction recoveries ranged from 97.9 to 101.6%. The validated HPLC method was successfully applied to a pharmacokinetic study of W‐1 in rats. Copyright © 2015 John Wiley & Sons, Ltd.