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Characterization of the constituents in rat plasma after oral administration of radix polygoni multiflori extracts by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry
Author(s) -
Lin Longfei,
Lin Hongmei,
Yin Xingbin,
Zhao Yang,
Xia Zhenwen,
Zhang Miao,
Li Xuechun,
Han Jing,
Qu Changhai,
Ni Jian
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3456
Subject(s) - quadrupole time of flight , chemistry , chromatography , mass spectrometry , radix (gastropod) , high performance liquid chromatography , time of flight mass spectrometry , quadrupole , plasma , analytical chemistry (journal) , tandem mass spectrometry , ionization , organic chemistry , physics , atomic physics , quantum mechanics , ion , botany , biology
An ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry method was established to detect as many constituents in rat plasma as possible after oral administration of Radix polygoni multiflori (RPM) extract. A C 18 column (150 × 2.0 mm, 4 µm) was adopted to separate the samples, and mass spectra were acquired in negative modes. The fingerprints of RPM extract were established, resulting in 39 components being detected. Among these compounds, 29 were identified by comparing the retention times and mass spectral data with those of reference standards and relevant references, and eight compounds were separated and detected in RPM for the first time. In vivo , 23 compounds were observed in dosed rat plasma, 16 of 23 compounds were indicated as prototype components of RPM, and seven compounds were predicted to be metabolites of RPM. A high‐speed and sensitive method was developed and was successfully utilized for screening and characterizing the ingredients and metabolites of RPM. Copyright © 2015 John Wiley & Sons, Ltd.