Premium
Development and application of an analytical method for curdione quantification in pregnant sprague–dawley rats by LC‐MS/MS
Author(s) -
Meng Xiang,
Zhang Ting,
Li Ying,
Pan Qi,
Jiang Juan,
Luo Yongwei,
Chong Liming,
Yang Yang,
Xu Sichong,
Zhou Li,
Sun Zuyue
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3449
Subject(s) - chemistry , chromatography , formic acid , glimepiride , detection limit , medicine , type 2 diabetes , diabetes mellitus , endocrinology
The vaginal administration route suffers from relatively low absorption efficiency, which may hinder the identification of the toxicokinetics of curdione in pregnant women. A sensitive analytical method for determining the plasma concentration of curdione was developed and applied in the determination of curdione in pregnant Sprague–Dawley rats as a simulated model. Glimepiride was used as an internal standard and chromatographic separation was achieved on a Capcell Pak C 18 MGIII column. A gradient elution profile with 0.5% formic acid (A)–0.5% formic acid–acetonitrile (B) was selected as mobile phase. The selected reaction monitoring mode was used for quantification based on the target fragment ions m / z 237.2 to m / z 135.1 for curdione and m / z 491.3 to m / z 352.1 for the glimepiride. The standard curve was linear over the range of 0.5–500 ng/mL for curdione in rat plasma and yielded a consistent peak pattern, even at the lower limit of quantitation of 0.5 ng/mL. The retention times of curdione and IS were 6.55 and 6.59 min, respectively. The mean recovery of curdione in rat plasma was 95.5–101.1%. The intra‐day and inter‐day precisions were between 2.35 and 9.08%. This LC‐MS/MS method provides a simple and sensitive means for determining the plasma concentration. Copyright © 2015 John Wiley & Sons, Ltd.