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A rapid and sensitive LC‐MS/MS method for quantification of 3,29‐dibenzoyl rarounitriol in rat plasma: application to a pharmacokinetic study
Author(s) -
Zhao Chengliang,
Zhang Nan,
Chen Bin,
Song Youxin,
Zhu Naiqiang,
Zhao Long,
Liu Changjian,
Meng Xiangwei
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3412
Subject(s) - chemistry , chromatography , ammonium acetate , electrospray ionization , formic acid , selected reaction monitoring , pharmacokinetics , calibration curve , detection limit , extraction (chemistry) , mass spectrometry , ethyl acetate , liquid chromatography–mass spectrometry , coefficient of variation , methanol , tandem mass spectrometry , high performance liquid chromatography , pharmacology , medicine , organic chemistry
A rapid, sensitive and high‐throughput liquid chromatography–tandem mass spectrometry was established and validated to assay the concentrations of 3,29‐dibenzoyl rarounitriol in rat plasma. Plasma samples were processed by liquid–liquid extraction with ethyl acetate and separated on a Hypersil Gold C 18 column (50 × 4.6 mm, 3 µm) at an isocratic flow rate of 0.5 mL/min using methanol–10 m m ammonium acetate–formic acid (90:10:0.1, v/v/v) as mobile phase. The total run time was 5 min for each sample. MS/MS detection was accomplished in selected reaction monitoring mode with positive electrospray ionization. The calibration curve was linear over the concentration range of 0.125–50 ng/mL with lower limit of quantification of 0.125 ng/mL. The intra‐ and inter‐day precisions were <10.1% in terms of coefficient of variation, and the accuracy was within ±11.7% in terms of relative error. The developed method was successfully applied to a pharmacokinetic study of 3,29‐dibenzoyl rarounitriol following intragastric administration of 3.65 mg/kg to Wistar rats. Copyright © 2015 John Wiley & Sons, Ltd.