Premium
Quantitation of VEGFR2 (vascular endothelial growth factor receptor) inhibitors – review of assay methodologies and perspectives
Author(s) -
Sharma Kuldeep,
Suresh P. S.,
Mullangi Ramesh,
Srinivas N. R.
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3370
Subject(s) - sunitinib , pazopanib , sorafenib , tyrosine kinase , bioanalysis , chemistry , kinase insert domain receptor , vascular endothelial growth factor , pharmacology , vegf receptors , receptor tyrosine kinase , cancer research , platelet derived growth factor receptor , kinase , cancer , vascular endothelial growth factor a , hepatocellular carcinoma , medicine , receptor , growth factor , biochemistry , chromatography
The introduction of small‐molecule tyrosine kinase VEGFR2 (vascular endothelial growth factor receptor) inhibitors has added another dimension in the treatment of several oncology indications as they offer a unique mechanism. The VEGFR2 inhibitors have demonstrated superior benefits in treating certain types of cancer, such as renal cell carcinoma and hepatocellular carcinoma, as a monotherapy option. Many of the approved VEGFR2 inhibitors have also shown promise when used in combination with other anticancer agents. There are numerous bioanalytical methods published for the analysis of VEGFR2 inhibitors in preclinical and clinical samples. This review covers VEGFR2 inhibitors such as sunitinib, sorafenib, pazopanib and JI‐101. In addition to providing a comprehensive review of the available methods for the above‐mentioned VRGFR2 inhibitors, it also provides information on assays that can simultaneously measure multiple tyrosine kinase inhibitors, including VEGFR2 molecules. Based on the review, the published methodologies using LC/MS‐MS or HPLC‐UV are adequate for the quantification of the VEGFR2 inhibitors and can easily be established in a modern day bioanalytical laboratory. The availability of a plethora of assays for multiple tyrosine kinase inhibitors makes it easy to analyze a panel of compounds to support either therapeutic drug monitoring and/or clinical pharmacokinetics. Copyright © 2014 John Wiley & Sons, Ltd.