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Effects of American ginseng on pharmacokinetics of 5‐fluorouracil in rats
Author(s) -
He YiSheng,
Sun Wei,
Wang ChongZhi,
Qi LianWen,
Yang Jie,
Li Ping,
Wen XiaoDong,
Yuan ChunSu
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3354
Subject(s) - pharmacokinetics , chemistry , ginseng , chromatography , electrospray ionization , half life , oral administration , mass spectrometry , pharmacology , medicine , alternative medicine , pathology
The pharmacokinetics of 5‐fluorouracil (5‐FU) in combination with or without American ginseng (seven‐consecutive days oral dose) in rats were evaluated using liquid chromatography–electrospray ionization–mass spectrometry (LC‐MS). Chromatographic separation was performed on a reverse LC column within a total run time of 6.5 min, which allowed for a relatively quick analysis. The limit of quantification for 5‐FU was 15 ng/mL and this method was linear over 15–50,000 ng/mL. This method supported stabilizing determination of the plasma concentration of 5‐FU over a period of 24 h. Precision both interday and intraday (coefficient of variation) was within 14% and accuracy (relative error) ranged from −5 to 14%. In view of the observed pharmacokinetic parameters, including maximum concentration, time to maximum concentration, area under the concentration–time curve (AUC), mean residence time, elimination half‐life and clearance, our results showed no significant differences in all of the pharmacokinetic parameters between the ginseng co‐treated group and 5‐FU alone group. Some increase in AUC was observed in 5‐FU plus ginseng group; however, the difference did not reach statistical significance compared with 5‐FU alone. It appeared that American ginseng administration did not significantly alter the kinetics of 5‐FU. More studies are still needed to confirm our results. Copyright © 2014 John Wiley & Sons, Ltd.