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Rapid identif ication and comparative analysis of chemical constituents in herbal medicine Fufang decoction by ultra‐high‐pressure liquid chromatography coupled with a hybrid linear ion trap–high‐resolution mass spectrometry
Author(s) -
Cao Gang,
Chen Xiaocheng,
Wu Xin,
Li Qinglin,
Zhang Hongyan
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3333
Subject(s) - decoction , chemistry , chromatography , orbitrap , mass spectrometry , anthraquinones , resolution (logic) , quadrupole ion trap , traditional medicine , ion trap , medicine , botany , artificial intelligence , computer science , biology
Abstract This study was conducted to reveal the relation between herbal medicine Fufang decoction and a single drug in terms of material base. Da‐Cheng‐Qi decoction (DCQD) was used as a model. Ultrahigh‐pressure liquid chromatography coupled with a hybrid linear ion trap–high‐resolution mass spectrometry (UHPLC‐LTQ‐Orbitrap) was applied to detect and identify the main chemical compounds. This technique was also employed to determine the different chemical components. Under optimized liquid chromatography and mass spectrometry conditions, 64 components, including iridoids, flavonoids, anthraquinones and coumarins, were separated and tentatively characterized in Da‐Cheng‐Qi decoction. After decoction, the contents of 18 compounds were markedly changed, and two components were no longer detected in Fufang decoction compared with single‐medicine decoction. The established method provided a good example for the rapid identification of complicated polar constituents in herbal medicine prescriptions. Copyright © 2014 John Wiley & Sons, Ltd.