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Recent bioanalytical methods for quantification of third‐generation cephalosporins using HPLC and LC‐MS(/MS) and their applications in pharmacokinetic studies
Author(s) -
Jin HyoEon,
Jin SuEon,
Maeng HanJoo
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3330
Subject(s) - bioanalysis , chemistry , cephalosporin , chromatography , cephalosporin antibiotic , high performance liquid chromatography , pharmacokinetics , mass spectrometry , liquid chromatography–mass spectrometry , tandem mass spectrometry , third generation , antibiotics , pharmacology , medicine , telecommunications , biochemistry , computer science
Third‐generation cephalosporins are semi‐synthetic antibiotics with enhanced activity against Gram‐negative organisms. The cephalosporins in biological samples are mostly determined using high‐performance liquid chromatography (HPLC) and HPLC–mass spectrometry (LC‐MS) or tandem mass spectrometry (LC‐MS/MS). In recent years, numerous bioanalytical methods have been developed to improve the sensitivity and specificity of cephalosporin quantification using the powerful LC‐MS/MS systems that are common in research laboratories. This review article presents recently developed bioanalytical methods by HPLC or LC‐MS(/MS) for 12 third‐generation cephalosporins, including five oral third‐generation cephalosporins, and further discusses their application in pharmacokinetic studies of animals and humans. Copyright © 2014 John Wiley & Sons, Ltd.