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Development and validation of a high‐performance liquid chromatography–tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma
Author(s) -
Park Semin,
Park ChulSoo,
Lee Sung Joong,
Cha Boseok,
Cho Young Ah,
Song Yi,
Yu Eun Ae,
Kim GonSup,
Jin Jong Sung,
Abd ElAty A. M.,
ElBanna H. A.,
Hacımüftüoğlu Ahmet,
Shim JaeHan,
Shin Sung Chul
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3322
Subject(s) - chemistry , chromatography , bupropion , escitalopram , quetiapine , human plasma , antidepressant , medicine , schizophrenia (object oriented programming) , pathology , neuroscience , biology , computer science , smoking cessation , hippocampus , programming language
In the present study, an effective high performance liquid chromatography–tandem mass spectrometric (HPLC/MS/MS) method was developed and validated to simultaneously determine bupropion (BUP), quetiapine (QUE) and escitalopram (ESC) in human plasma using carbidopa as the internal standard. Chromatographic separation was achieved on a Waters Sun Fire C 18 column using reversed‐phase chromatography. The MS/MS experiment was performed in positive ion multiple reaction monitoring mode to produce product ions of m/z 240.3 → 184.2 for BUP, 384.2 → 253.1 for QUE, 325.3 → 109.3 for ESC and 227.2 → 181.2 for the internal standard. The method showed good linearity ( R 2 ≥ 0.997), precision (relative standard deviation ≤7.5%), satisfactory intra‐ and interday accuracy (88.4–113.0%) and acceptable extraction recovery (87.2–115.0%), matrix effect (84.5.5−108.7%) and stability (92.3−103.5%). The method was successfully applied to determine the concentrations of BUP, QUE and ESC in human plasma samples. Copyright © 2014 John Wiley & Sons, Ltd.